Green Indicator Plates
Jeff Lawrence (Z51)
(NOTE: This section describes classic green plates, which have now been superceded by "Mint Green Plates")
828 g Bacto-Tryptone (Difco)
105 g Yeast Extract (Difco)
516 g NaCl
1551 g Agar
6.8 g Aniline Blue (C32H27N3O9S3·2Na)
64.4 g Alizarin Yellow G (C13H8N3NaO5)
1 Place ingredients into a plastic tub and shake vigorously to distribute dyes well.
Normal recipe - 4 L of green plates:
2. Autoclave 116 g Plate Mix in 4L water.
3. When cool enough to pour, add 67.2 mL of 50% Glucose.
Make Dye Stock as:
6.5 mg/ml Aniline Blue 1.3 g Aniline Blue
62.2 mg/ml Alizarin Yellow 12.4 g Alizarin Yellow
200 mL Water
Combine and Autoclave:
8 g Bacto-Tryptone (Difco)
1 g Yeast Extract (Difco)
5 g NaCl
15 g Agar
10 mL Dye Stock
990 mL Water
After cooling, add 16.8 mL of 50% Glucose and pour.
Last Update: Thursday June 19 2014
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