Universal Chloramphenicol Resistance Cassette
Sequence of universal CmR-cassette template.
Made by amplifying pACYC184 with TP928 (ul1-cmr), which binds upstream
of cat promoter and introduces the UL1 universal binding site at the
upstream end of the amplified DNA, and TP929 (ur1-cmr), which binds
downstream of putative transcriptional stop following cat and
introduces the UR1 universal binding site at the downstream end of the
amplified DNA.
UL1 1 21
promoter 214 240
potential msg start 247
cat (camR) 307 966
UR1 1204 1224
1 CACCAAACAC CCCCCAAAAC CAAGCCACTG GAGCACCTCA AAAACACCAT
51 CATACACTAA ATCAGTAAGT TGGCAGCATC ACCCGACGCA CTTTGCGCCG
101 AATAAATACC TGTGACGGAA GATCACTTCG CAGAATAAAT AAATCCTGGT
151 GTCCCTGTTG ATACCGGGAA GCCCTGGGCC AACTTTTGGC GAAAATGAGA
201 CGTTGATCGG CACGTAAGAG GTTCCAACTT TCACCATAAT GAAATAAGAT
251 CACTACCGGG CGTATTTTTT GAGTTATCGA GATTTTCAGG AGCTAAGGAA
301 GCTAAAATGG AGAAAAAAAT CACTGGATAT ACCACCGTTG ATATATCCCA
351 ATGGCATCGT AAAGAACATT TTGAGGCATT TCAGTCAGTT GCTCAATGTA
401 CCTATAACCA GACCGTTCAG CTGGATATTA CGGCCTTTTT AAAGACCGTA
451 AAGAAAAATA AGCACAAGTT TTATCCGGCC TTTATTCACA TTCTTGCCCG
501 CCTGATGAAT GCTCATCCGG AATTCCGTAT GGCAATGAAA GACGGTGAGC
551 TGGTGATATG GGATAGTGTT CACCCTTGTT ACACCGTTTT CCATGAGCAA
601 ACTGAAACGT TTTCATCGCT CTGGAGTGAA TACCACGACG ATTTCCGGCA
651 GTTTCTACAC ATATATTCGC AAGATGTGGC GTGTTACGGT GAAAACCTGG
701 CCTATTTCCC TAAAGGGTTT ATTGAGAATA TGTTTTTCGT CTCAGCCAAT
751 CCCTGGGTGA GTTTCACCAG TTTTGATTTA AACGTGGCCA ATATGGACAA
801 CTTCTTCGCC CCCGTTTTCA CCATGGGCAA ATATTATACG CAAGGCGACA
851 AGGTGCTGAT GCCGCTGGCG ATTCAGGTTC ATCATGCCGT CTGTGATGGC
901 TTCCATGTCG GCAGAATGCT TAATGAATTA CAACAGTACT GCGATGAGTG
951 GCAGGGCGGG GCGTAATTTT TTTAAGGCAG TTATTGGTGC CCTTAAACGC
1001 CTGGTGCTAC GCCTGAATAA GTGATAATAA GCGGATGAAT GGCAGAAATT
1051 CGAAAGCAAA TTCGACCCGG TCGTCGGTTC AGGGCAGGGT CGTTAAATAG
1101 CCGCTTATGT CTATTGCTGG TTTACCGGTT TATTGACTAC CGGAAGCAGT
1151 GTGACCGTGT GCTTCTCAAA TGCCTGAGGC CAGTTTGCTC AGGCTCTCCC
1201 CGTGTGGTGT GGTGTGGTTG TGTG