Universal Chloramphenicol Resistance Cassette
(Standard template in strain TT23216)

Sequence of universal CmR-cassette template.

Made by amplifying pACYC184 with TP928 (ul1-cmr), which binds upstream
of cat promoter and introduces the UL1 universal binding site at the
upstream end of the amplified DNA, and TP929 (ur1-cmr), which binds
downstream of putative transcriptional stop following aaaC1 and
introduces the UR1 universal binding site at the downstream end of the
amplified DNA.

  UL1			1	21
  promoter		214	240
  potential msg start	247
  aaaC1 (camR)		307	966
  UR1			1204	1224

       1  CACCAAACAC CCCCCAAAAC CAAGCCACTG GAGCACCTCA AAAACACCAT 
      51  CATACACTAA ATCAGTAAGT TGGCAGCATC ACCCGACGCA CTTTGCGCCG 
     101  AATAAATACC TGTGACGGAA GATCACTTCG CAGAATAAAT AAATCCTGGT 
     151  GTCCCTGTTG ATACCGGGAA GCCCTGGGCC AACTTTTGGC GAAAATGAGA 
     201  CGTTGATCGG CACGTAAGAG GTTCCAACTT TCACCATAAT GAAATAAGAT 
     251  CACTACCGGG CGTATTTTTT GAGTTATCGA GATTTTCAGG AGCTAAGGAA 
     301  GCTAAAATGG AGAAAAAAAT CACTGGATAT ACCACCGTTG ATATATCCCA 
     351  ATGGCATCGT AAAGAACATT TTGAGGCATT TCAGTCAGTT GCTCAATGTA 
     401  CCTATAACCA GACCGTTCAG CTGGATATTA CGGCCTTTTT AAAGACCGTA 
     451  AAGAAAAATA AGCACAAGTT TTATCCGGCC TTTATTCACA TTCTTGCCCG 
     501  CCTGATGAAT GCTCATCCGG AATTCCGTAT GGCAATGAAA GACGGTGAGC 
     551  TGGTGATATG GGATAGTGTT CACCCTTGTT ACACCGTTTT CCATGAGCAA 
     601  ACTGAAACGT TTTCATCGCT CTGGAGTGAA TACCACGACG ATTTCCGGCA 
     651  GTTTCTACAC ATATATTCGC AAGATGTGGC GTGTTACGGT GAAAACCTGG 
     701  CCTATTTCCC TAAAGGGTTT ATTGAGAATA TGTTTTTCGT CTCAGCCAAT 
     751  CCCTGGGTGA GTTTCACCAG TTTTGATTTA AACGTGGCCA ATATGGACAA 
     801  CTTCTTCGCC CCCGTTTTCA CCATGGGCAA ATATTATACG CAAGGCGACA 
     851  AGGTGCTGAT GCCGCTGGCG ATTCAGGTTC ATCATGCCGT CTGTGATGGC 
     901  TTCCATGTCG GCAGAATGCT TAATGAATTA CAACAGTACT GCGATGAGTG 
     951  GCAGGGCGGG GCGTAATTTT TTTAAGGCAG TTATTGGTGC CCTTAAACGC 
    1001  CTGGTGCTAC GCCTGAATAA GTGATAATAA GCGGATGAAT GGCAGAAATT 
    1051  CGAAAGCAAA TTCGACCCGG TCGTCGGTTC AGGGCAGGGT CGTTAAATAG 
    1101  CCGCTTATGT CTATTGCTGG TTTACCGGTT TATTGACTAC CGGAAGCAGT 
    1151  GTGACCGTGT GCTTCTCAAA TGCCTGAGGC CAGTTTGCTC AGGCTCTCCC 
    1201  CGTGTGGTGT GGTGTGGTTG TGTG

Last Update: Monday, 14-Nov-2016 14:55:55 PST
This page has been viewed 122 times since 14-Nov-2016.
Eric Kofoid eckofoid at ucdavis.edu