Universal Gentamycin Resistance Cassette
(Standard template in strain TT24691)

Sequence of universal gent-cassette template.  

Made by exact replacement of camR coding region of uni-cmr-cassette 
with the coding region of gene aaaC1 (from Tn21 -- see gb:X15852), 
encoding gentamycin resistance.

  UL1			1	21
  promoter		214	240
  potential msg start	247
  aaaC1 (gentR)		307	840
  UR1			1078	1098

       1  CACCAAACAC CCCCCAAAAC CAAGCCACTG GAGCACCTCA AAAACACCAT 
      51  CATACACTAA ATCAGTAAGT TGGCAGCATC ACCCGACGCA CTTTGCGCCG 
     101  AATAAATACC TGTGACGGAA GATCACTTCG CAGAATAAAT AAATCCTGGT 
     151  GTCCCTGTTG ATACCGGGAA GCCCTGGGCC AACTTTTGGC GAAAATGAGA 
     201  CGTTGATCGG CACGTAAGAG GTTCCAACTT TCACCATAAT GAAATAAGAT 
     251  CACTACCGGG CGTATTTTTT GAGTTATCGA GATTTTCAGG AGCTAAGGAA 
     301  GCTAAAATGT TACGCAGCAG CAACGATGTT ACGCAGCAGG GCAGTCGCCC 
     351  TAAAACAAAG TTAGGTGGCT CAAGTATGGG CATCATTCGC ACATGTAGGC 
     401  TCGGCCCTGA CCAAGTCAAA TCCATGCGGG CTGCTCTTGA TCTTTTCGGT 
     451  CGTGAGTTCG GAGACGTAGC CACCTACTCC CAACATCAGC CGGACTCCGA 
     501  TTACCTCGGG AACTTGCTCC GTAGTAAGAC ATTCATCGCG CTTGCTGCCT 
     551  TCGACCAAGA AGCGGTTGTT GGCGCTCTCG CGGCTTACGT TCTGCCCAGG 
     601  TTTGAGCAGC CGCGTAGTGA GATCTATATC TATGATCTCG CAGTCTCCGG 
     651  CGAGCACCGG AGGCAGGGCA TTGCCACCGC GCTCATCAAT CTCCTCAAGC 
     701  ATGAGGCCAA CGCGCTTGGT GCTTATGTGA TCTACGTGCA AGCAGATTAC 
     751  GGTGACGATC CCGCAGTGGC TCTCTATACA AAGTTGGGCA TACGGGAAGA 
     801  AGTGATGCAC TTTGATATCG ACCCAAGTAC CGCCACCTAA TTTTTTTAAG 
     851  GCAGTTATTG GTGCCCTTAA ACGCCTGGTG CTACGCCTGA ATAAGTGATA 
     901  ATAAGCGGAT GAATGGCAGA AATTCGAAAG CAAATTCGAC CCGGTCGTCG 
     951  GTTCAGGGCA GGGTCGTTAA ATAGCCGCTT ATGTCTATTG CTGGTTTACC 
    1001  GGTTTATTGA CTACCGGAAG CAGTGTGACC GTGTGCTTCT CAAATGCCTG 
    1051  AGGCCAGTTT GCTCAGGCTC TCCCCGTGTG GTGTGGTGTG GTTGTGTG

Last Update: Monday, 14-Nov-2016 15:00:43 PST
This page has been viewed 332 times since 14-Nov-2016.
Eric Kofoid eckofoid at ucdavis.edu