Universal Spectinomycin Resistance Cassette
(Standard template in strain TT24693)

Sequence of universal spec-cassette template.

Made by precise replacement of cat gene of uni-cmr-cassette with
spectinomycin resistance gene, aadA.

UL1			1	21
promoter		214	240
potential msg start	247
aadA (specR)		307	1317
UR1			1555	1575

       1  CACCAAACAC CCCCCAAAAC CAAGCCACTG GAGCACCTCA AAAACACCAT 
      51  CATACACTAA ATCAGTAAGT TGGCAGCATC ACCCGACGCA CTTTGCGCCG 
     101  AATAAATACC TGTGACGGAA GATCACTTCG CAGAATAAAT AAATCCTGGT 
     151  GTCCCTGTTG ATACCGGGAA GCCCTGGGCC AACTTTTGGC GAAAATGAGA 
     201  CGTTGATCGG CACGTAAGAG GTTCCAACTT TCACCATAAT GAAATAAGAT 
     251  CACTACCGGG CGTATTTTTT GAGTTATCGA GATTTTCAGG AGCTAAGGAA 
     301  GCTAAAATGC GCTCACGCAA CTGGTCCAGA ACCTTGACCG AACGCAGCGG 
     351  TGGTAACGGC GCAGTGGCGG TTTTCATGGC TTGTTATGAC TGTTTTTTTG 
     401  GGGTACAGTC TATGCCTCGG GCATCCAAGC AGCAAGCGCG TTACGCCGTG 
     451  GGTCGATGTT TGATGTTATG GAGCAGCAAC GATGTTACGC AGCAGGGCAG 
     501  TCGCCCTAAA ACAAAGTTAA ACATCATGAG GGAAGCGGTG ATCGCCGAAG 
     551  TATCGACTCA ACTATCAGAG GTAGTTGGCG TCATCGAGCG CCATCTCGAA 
     601  CCGACGTTGC TGGCCGTACA TTTGTACGGC TCCGCAGTGG ATGGCGGCCT 
     651  GAAGCCACAC AGTGATATTG ATTTGCTGGT TACGGTGACC GTAAGGCTTG 
     701  ATGAAACAAC GCGGCGAGCT TTGATCAACG ACCTTTTGGA AACTTCGGCT 
     751  TCCCCTGGAG AGAGCGAGAT TCTCCGCGCT GTAGAAGTCA CCATTGTTGT 
     801  GCACGACGAC ATCATTCCGT GGCGTTATCC AGCTAAGCGC GAACTGCAAT 
     851  TTGGAGAATG GCAGCGCAAT GACATTCTTG CAGGTATCTT CGAGCCAGCC 
     901  ACGATCGACA TTGATCTGGC TATCTTGCTG ACAAAAGCAA GAGAACATAG 
     951  CGTTGCCTTG GTAGGTCCAG CGGCGGAGGA ACTCTTTGAT CCGGTTCCTG 
    1001  AACAGGATCT ATTTGAGGCG CTAAATGAAA CCTTAACGCT ATGGAACTCG 
    1051  CCGCCCGACT GGGCTGGCGA TGAGCGAAAT GTAGTGCTTA CGTTGTCCCG 
    1101  CATTTGGTAC AGCGCAGTAA CCGGCAAAAT CGCGCCGAAG GAGGTCGCTG 
    1151  CCGACTGGGC AATGGAGCGC CTGCCGGCCC AGTATCAGCC CGTCATACGT 
    1201  GAAGCTAGAC AGGCTTATCT TGGACAAGAA GAAGATCGCT TGGCCTCGCG 
    1251  CGCAGATCAG TTGGAAGAAT TTGTCCACTA CGTGAAAGGC GAGATCACCA 
    1301  AGGTAGTCGG CAAATAATTT TTTTAAGGCA GTTATTGGTG CCCTTAAACG 
    1351  CCTGGTGCTA CGCCTGAATA AGTGATAATA AGCGGATGAA TGGCAGAAAT 
    1401  TCGAAAGCAA ATTCGACCCG GTCGTCGGTT CAGGGCAGGG TCGTTAAATA 
    1451  GCCGCTTATG TCTATTGCTG GTTTACCGGT TTATTGACTA CCGGAAGCAG 
    1501  TGTGACCGTG TGCTTCTCAA ATGCCTGAGG CCAGTTTGCT CAGGCTCTCC 
    1551  CCGTGTGGTG TGGTGTGGTT GTGTG

Last Update: Monday, 14-Nov-2016 15:02:47 PST
This page has been viewed 452 times since 14-Nov-2016.
Eric Kofoid eckofoid at ucdavis.edu