Universal Zeocin Resistance Cassette
(Standard template in strain TT24692)

Sequence of universal zeo-cassette template.

Made by exact replacement of camR coding region of uni-cmr-cassette 
with the coding region of gene ble, encoding zeocin resistance.
Use strain TT24692 as template for this cassette.
  
  UL1			1	21
  promoter		214	240
  potential msg start	247
  ble (zeoR)		307	681
  UR1			919	939

       1  CACCAAACAC CCCCCAAAAC CAAGCCACTG GAGCACCTCA AAAACACCAT 
      51  CATACACTAA ATCAGTAAGT TGGCAGCATC ACCCGACGCA CTTTGCGCCG 
     101  AATAAATACC TGTGACGGAA GATCACTTCG CAGAATAAAT AAATCCTGGT 
     151  GTCCCTGTTG ATACCGGGAA GCCCTGGGCC AACTTTTGGC GAAAATGAGA 
     201  CGTTGATCGG CACGTAAGAG GTTCCAACTT TCACCATAAT GAAATAAGAT 
     251  CACTACCGGG CGTATTTTTT GAGTTATCGA GATTTTCAGG AGCTAAGGAA 
     301  GCTAAAATGG CCAAGTTGAC CAGTGCCGTT CCGGTGCTCA CCGCGCGCGA 
     351  CGTCGCCGGA GCGGTCGAGT TCTGGACCGA CCGGCTCGGG TTCTCCCGGG 
     401  ACTTCGTGGA GGACGACTTC GCCGGTGTGG TCCGGGACGA CGTGACCCTG 
     451  TTCATCAGCG CGGTCCAGGA CCAGGTGGTG CCGGACAACA CCCTGGCCTG 
     501  GGTGTGGGTG CGCGGCCTGG ACGAGCTGTA CGCCGAGTGG TCGGAGGTCG 
     551  TGTCCACGAA CTTCCGGGAC GCCTCCGGGC CGGCCATGAC CGAGATCGGC 
     601  GAGCAGCCGT GGGGGCGGGA GTTCGCCCTG CGCGACCCGG CCGGCAACTG 
     651  CGTGCACTTC GTGGCCGAGG AGCAGGACTG ATTTTTTTAA GGCAGTTATT 
     701  GGTGCCCTTA AACGCCTGGT GCTACGCCTG AATAAGTGAT AATAAGCGGA 
     751  TGAATGGCAG AAATTCGAAA GCAAATTCGA CCCGGTCGTC GGTTCAGGGC 
     801  AGGGTCGTTA AATAGCCGCT TATGTCTATT GCTGGTTTAC CGGTTTATTG 
     851  ACTACCGGAA GCAGTGTGAC CGTGTGCTTC TCAAATGCCT GAGGCCAGTT 
     901  TGCTCAGGCT CTCCCCGTGT GGTGTGGTGT GGTTGTGTG

Last Update: Wednesday, 04-Oct-2017 10:55:19 PDT
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Eric Kofoid eckofoid at ucdavis.edu