DNA Purification - Freezing Method


Jeff Lawrence

1. Remove gel slice contain DNA fragment and place in 10 volumes of:
	300 mM	NaOAc, pH 7.0	(300 mL 1 M NaOAC, pH 7.0)
	1 mM	EDTA		(2 mL	500 mM EDTA, pH 8.0)
				(698 mL	ddH20)
2. Incubate at 22° for 30 min. Transfer gel slice to a fresh tube.

3. Place tube in a Dry Ice/Ethanol bath for 5 min.

4. Puncture the bottom of a 0.5 mL microcentrifuge tube with a needle. Place the gel slice into this tube. Place this tube inside a 1.5 mL microcentrifuge tube.

5. Centrifuge for 15 min.

6. Collect the Eluent from the 1.5 mL eppendorf tube. Extract and precipitate the DNA.


Last Update: Thursday, 19-Jun-2014 11:47:03 PDT
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Eric Kofoid eckofoid at ucdavis.edu