DNA Purification - Manual Detritylation of Oligonucleotides


Jeff Lawrence

1. If the trityl group is not removed from a newly-synthesized oligonucleotide, it may interfere with polymerization reactions.

2. Evaporate the oligonucleotide to dryness.

3. Add 300 µL 80% Acetic acid. Incubate at 22° for 20 min.

4. Add 300 µL 100% Ethanol. Evaporate to dryness.

5. If a white precipitate forms from volatile salts: resuspend in 300 µL ddH2O, add 300 µL 100% Ethanol. Evaporate to dryness.

6. Resuspend oligonucleotide in 500 µL ddH2O.