DNA Reaction - Kinasing


Jeff Lawrence

1. Mix the following reagents: 
	11 µL	ddH2O
	1 µl	20 pmol of oligonucleotide.
	2 µL	10X Kinase Buffer
	1 µL	T4 DNA Kinase, @10 units/µL
	5 µL	g-32P-ATP
2. Incubate at 37° for 15-30 min.

3. Incubate at 70° for 5 min to inactivate enzyme.

10X Kinase Buffer is: 
	50 mL	1 M Tris, pH 8.0
	10 mL	1 M MgCl2
	5 mL	1 M DTT
	935 mL	ddH2O