Salmonella typhimurium Mud-P22 strains


Jeff Lawrence (Z20)
	Kit#	Minute	NB#	TT#	Genotype		Pac	MTL#
	1	00.0	110	15223	thr-469::MudP		A	2P
	2	3.5	111	15226	nadC218::MudP		A	4P
	3	8.5	104	15230	proC693::MudP		A	8P
	4	12.0	155	15232	purE2154::MudQ		A	9Q
	5	17.0	136	15238	nadA219::MudP		A	16P
	6	21.5	177	15239	putA1019::MudQ		A	17Q
	7	28.5	195	15243	aroD561::MudQ		B	19Q
	8	33.0	152	15246	pyrF2690::MudP		B	20P
	9	35.9	204	15630	tre-152::MudP		A	37P
	10	40.5	193	15250	zea-3666::MudP		B	40P
	11	40.5	196	15249	zea-3666::MudQ		A	40Q
	12	50.0	126	15258	cysA1585::MudP		B	11P
	13	52.0	206	15632	guaAB5641::MudQ		A	7Q
	14	52.0	169	15255	guaAB5641::MudP		B	7P
	15	57.0	186	15261	proU1884::MudP		A	36P
	16	57.0	202	15628	proU1884::MudQ		B	36Q
	17	60.0	165	15263	cysHIJ1574::MudP	B	23P
	18	62.7	217	16707	zgc-3715::MudQ		B	TDL38
	19	65.0	222	16709	zgf-3716::MudQ		B	TDL40
	20	68.5	299	17191	zgi-3717::MudQ		B	TDL71
	21	68.5	298	17190	zgi-3717::MudP		A	TDL71
	22	73.6	300	17165	envZ1005::MudQ		A	TDL73
	23	73.6	189	15265	envZ1005::MudP		B	35P
	24	79.7	103	15267	pyrE2419::MudP		A	29P
	25	83.0	105	15269	ilvA2648::MudP		A	30P
	26	86.7	172	15273	pnuE41::MudQ		A	28Q
	27	86.7	171	15272	pnuE41::MudP		B	28P
	28	93.0	107	15276	melAB396::MudP		A	32P
	29	97.0	212	15638	zjh-3725::MudP		A	59P
	30	97.0	207	15633	zjh-3725::MudQ		B	59Q
	
	A=clockwise
	B=counterclockwise
Use 2 µL of each lysate for auxotrophic mapping on minimal media
and 5-10 µL of each lysate for TetS mapping on Bochner plates.



Last Update: Thursday, 19-Jun-2014 11:54:50 PDT
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Eric Kofoid eckofoid at ucdavis.edu