T2 Buffer


Jeff Lawrence (Z53)

1. Add, in order, to 2 L water:
	15 g	K2SO4
	12 g	NaCl
	4.5 g	KH2PO4
	9 g	Na2HPO4
2. Add water to 3 L; autoclave in 100 mL aliquots for 20 minutes and allow to cool.

3. To each 100 mL bottle add the following:
	0.1ml	1M MgSO4
	1 mL	.1% gelatin
	1 mL	.01M CaCl2

Last Update: Thursday, 19-Jun-2014 11:56:25 PDT
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Eric Kofoid eckofoid at ucdavis.edu