First String MudP22s


Kit # Minute NB # TT# Genotype of Mud Insertion Pac BKG MTL #
1 00.0 110 15223 thr-469::MudP A MS1868 2P
2 3.5 111 15226 nadC218::MudP A MS1868 4P
3 8.5 104 15230 proC693::MudP A MS1868 8P
4 12.0 155 15232 purE2154::MudQ A MS1868 9Q
5 17.0 136 15238 nadA219::MudP A MS1868 16P
6 21.5 177 15239 putA1019::MudQ A MS1868 17Q
7 28.5 195 15243 aroD561::MudQ B MS1868 19Q
8 33.0 152 15246 pyrF2690::MudP B MS1868 20P
9 35.9 204 15630 tre-152::MudP A MS1868 37P
10 40.5 193 15250 zea-3666::MudP B MS1868 40P
11 40.5 196 15249 zea-3666::MudQ A MS1868 40Q
12 50.0 126 15258 cysA1585::MudP B MS1868 11P
13 52.0 206 15632 guaAB5641::MudQ A MS1868 7Q
14 52.0 169 15255 guaAB5641::MudP B MS1868 7P
15 57.0 186 15261 proU1884::MudP A MS1868 36P
16 57.0 202 15628 proU1884::MudQ B MS1868 36Q
17 60.0 165 15263 cysHIJ1574::MudP B MS1868 23P
18 62.7 217 16707 zgc-3715::Mud Q B MS1868 TDL38
19 65.0 222 16709 zgf-3716::MudQ B MS1868 TDL40
20 68.5 299 17191 zgi-3717::MudQ B MS1868 TDL71
21 68.5 298 17190 zgi-3717::MudP A MS1868 TDL71
22 73.6 300 17165 envZ1005::MudQ A MS1868 TDL73
23 73.6 189 15265 envZ1005::MudP B MS1868 35P
24 79.7 103 15267 pyrE2419::MudP A MS1868 29P
25 83.0 105 15269 ilvA2648::MudP A MS1868 30P
26 86.7 172 15273 pnuE41::MudQ A MS1868 28Q
27 86.7 171 15272 pnuE41::MudP B MS1868 28P
28 93.0 107 15276 melAB396::MudP A MS1868 32P
29 97.0 212 15638 zjh-3725::MudP A MS1868 59P
30 97.0 207 15633 zjh-3725::MudQ B MS1868 59Q
A=clockwise
B=counterclockwise

Use 2ml of each lysate for auxotrophic mapping on minimal media
and 5-10ml of each lysate for tets mapping on Bochner plates.

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Last Update: Thursday June 19 2014

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Eric Kofoid eckofoid at ucdavis.edu