(Adapted from Davis, Botstein and Roth (1980), Appendix III, p 207.)

Stock solutions are 200x except where indicated. "Low" is the limiting 
concentration at which auxotrophs will form tiny colonies distinguishable 
from wild type. "F", filter sterilize; "A", autoclave.

                       Plate   Stock   "Low"   "Low"
                       conc.    sol.   conc.    ml/L
      Nutrient         (mM)     (%)    (mM)     (%)    Remarks
      ===============  ======  ======  ======  ======  ==============
      Adenine           5.0    1.35    0.001     -     F; 0.1 N HC1
      Adenosine         5.0    2.67    0.001     -     A
      Alanine           0.47   0.84      -       -     A
      Arginine          0.6    2.53    0.01    0.86    A
      Asparagine        0.32   0.84      -       -     F
      Aspartate-K       0.3    1.0       -       -     F
      Biotin            0.1    0.49      -       -     A
      Cysteine          0.3    0.73      -       -     F
      Diaminopimelate   0.1    0.38      -       -     A; (DAP)
      Glutamate-Na      5.0    8.5       -       -     F; 100xstock
      Glutamine         5.0    14.6      -       -     A
      Glycine           0.13   0.2       -       -     A
      Guanine           0.3    0.91      -       -     A; 1 N HC1
      Guanosine         0.3    1.7       -       -     A
      Histidine         0.1    0.31    0.005   0.25    A
      Histidinol        1.0    4.28      -       -     A
      Isoleucine        0.3    0.79      -       -     A
      Leucine           0.3    0.79    0.005   0.086   A
      Lysine            0.3    1.1     0.005   0.086   A
      Methionine        0.3    0.9       -     0.086   A
      Nicotinic acid    0.1    0.25      -       -     A
      Pantothenate-Ca   0.1    0.48      -       -     A
      Phenylalanine     0.3    0.99      -       -     A; 0.01 N HCl
      Proline           2.0    4.6     0.002   0.005   A
      Pyridoxine HCl    0.1    0.41      -       -     A
      Serine            4.0    8.4     0.01    0.0125  A
      Thiamine          0.05   0.337     -       -     A
      Threonine         0.3    0.71      -       -     A
      Thymine           0.32   0.81      -       -     A
      Tryptophan        0.1    0.41      -       -     F
      Tyrosine          0.1    0.36      -       -     F
      Uracil            0.1    0.224   0.003   0.15    A
      Uridine           0.1    0.488   0.003     -     A
      Valine            0.3    0.7       -       -     A
      EGTA             10.0    2 M       -       -     A; neutralize

Last Update: Sunday, 26-Jan-2014 18:40:16 PST
This page has been viewed 420 times.
Eric Kofoid eckofoid at ucdavis.edu