Universal Gentamycin Resistance Cassette
(Standard template in strain TT24691)
Sequence of universal gent-cassette template.
Made by exact replacement of camR coding region of uni-cmr-cassette
with the coding region of gene aaaC1 (from Tn21 -- see gb:X15852),
encoding gentamycin resistance. Use strain TT24691 as template
for this cassette.
UL1 1 21
promoter 214 240
potential msg start 247
aaaC1 (gentR) 307 840
UR1 1078 1098
1 CACCAAACAC CCCCCAAAAC CAAGCCACTG GAGCACCTCA AAAACACCAT
51 CATACACTAA ATCAGTAAGT TGGCAGCATC ACCCGACGCA CTTTGCGCCG
101 AATAAATACC TGTGACGGAA GATCACTTCG CAGAATAAAT AAATCCTGGT
151 GTCCCTGTTG ATACCGGGAA GCCCTGGGCC AACTTTTGGC GAAAATGAGA
201 CGTTGATCGG CACGTAAGAG GTTCCAACTT TCACCATAAT GAAATAAGAT
251 CACTACCGGG CGTATTTTTT GAGTTATCGA GATTTTCAGG AGCTAAGGAA
301 GCTAAAATGT TACGCAGCAG CAACGATGTT ACGCAGCAGG GCAGTCGCCC
351 TAAAACAAAG TTAGGTGGCT CAAGTATGGG CATCATTCGC ACATGTAGGC
401 TCGGCCCTGA CCAAGTCAAA TCCATGCGGG CTGCTCTTGA TCTTTTCGGT
451 CGTGAGTTCG GAGACGTAGC CACCTACTCC CAACATCAGC CGGACTCCGA
501 TTACCTCGGG AACTTGCTCC GTAGTAAGAC ATTCATCGCG CTTGCTGCCT
551 TCGACCAAGA AGCGGTTGTT GGCGCTCTCG CGGCTTACGT TCTGCCCAGG
601 TTTGAGCAGC CGCGTAGTGA GATCTATATC TATGATCTCG CAGTCTCCGG
651 CGAGCACCGG AGGCAGGGCA TTGCCACCGC GCTCATCAAT CTCCTCAAGC
701 ATGAGGCCAA CGCGCTTGGT GCTTATGTGA TCTACGTGCA AGCAGATTAC
751 GGTGACGATC CCGCAGTGGC TCTCTATACA AAGTTGGGCA TACGGGAAGA
801 AGTGATGCAC TTTGATATCG ACCCAAGTAC CGCCACCTAA TTTTTTTAAG
851 GCAGTTATTG GTGCCCTTAA ACGCCTGGTG CTACGCCTGA ATAAGTGATA
901 ATAAGCGGAT GAATGGCAGA AATTCGAAAG CAAATTCGAC CCGGTCGTCG
951 GTTCAGGGCA GGGTCGTTAA ATAGCCGCTT ATGTCTATTG CTGGTTTACC
1001 GGTTTATTGA CTACCGGAAG CAGTGTGACC GTGTGCTTCT CAAATGCCTG
1051 AGGCCAGTTT GCTCAGGCTC TCCCCGTGTG GTGTGGTGTG GTTGTGTG
Last Update: Wednesday October 04 2017
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Eric Kofoid
eckofoid at ucdavis.edu