Universal Kanamycin Resistance Cassette (CamR Promoter)
(Standard template in strain TT23664)

Sequence of universal KnR-cassette template, pBR322 promoter.

Amplified from an aphA-2 insertion into pBR322.
Use strain TT23664 as template for this cassette. 

UL1: 1->21
pBR322 tet promoter and message start: 22->56
aphA-2: 90->884
UR1: 885->905.

       1  CACCAAACAC CCCCCAAAAC CTTGACAGCT TATCATCGAT AAGCTTTAAT 
      51  GCGGTAGTTT AGGTGAGGAC AGGATGAGGA TCGTTTCGCA TGATTGAACA 
     101  AGATGGATTG CACGCAGGTT CTCCGGCCGC TTGGGTGGAG AGGCTATTCG 
     151  GCTATGACTG GGCACAACAG ACAATCGGCT GCTCTGATGC CGCCGTGTTC 
     201  CGGCTGTCAG CGCAGGGGCG CCCGGTTCTT TTTGTCAAGA CCGACCTGTC 
     251  CGGTGCCCTG AATGAACTGC AGGACGAGGC AGCGCGGCTA TCGTGGCTGG 
     301  CCACGACGGG CGTTCCTTGC GCAGCTGTGC TCGACGTTGT CACTGAAGCG 
     351  GGAAGGGACT GGCTGCTATT GGGCGAAGTG CCGGGGCAGG ATCTCCTGTC 
     401  ATCTCACCTT GCTCCTGCCG AGAAAGTATC CATCATGGCT GATGCAATGC 
     451  GGCGGCTGCA TACGCTTGAT CCGGCTACCT GCCCATTCGA CCACCAAGCG 
     501  AAACATCGCA TCGAGCGAGC ACGTACTCGG ATGGAAGCCG GTCTTGTCGA 
     551  TCAGGATGAT CTGGACGAAG AGCATCAGGG GCTCGCGCCA GCCGAACTGT 
     601  TCGCCAGGCT CAAGGCGCGC ATGCCCGACG GCGAGGATCT CGTCGTGACC 
     651  CATGGCGATG CCTGCTTGCC GAATATCATG GTGGAAAATG GCCGCTTTTC 
     701  TGGATTCATC GACTGTGGCC GGCTGGGTGT GGCGGACCGC TATCAGGACA 
     751  TAGCGTTGGC TACCCGTGAT ATTGCTGAAG AGCTTGGCGG CGAATGGGCT 
     801  GACCGCTTCC TCGTGCTTTA CGGTATCGCC GCTCCCGATT CGCAGCGCAT 
     851  CGCCTTCTAT CGCCTTCTTG ACGAGTTCTT CTGAGTGGTG TGGTGTGGTT 
     901  GTGTG

Last Update: Wednesday October 04 2017
This page has been viewed Failed to execute CGI : Win32 Error Code = 2
times since 14-Nov-2016.
Eric Kofoid eckofoid at ucdavis.edu