Universal Kanamycin Resistance Cassette (CamR Promoter)
(Standard template in strain TT23664)
Sequence of universal KnR-cassette template, pBR322 promoter.
Amplified from an aphA-2 insertion into pBR322.
Use strain TT23664 as template for this cassette.
UL1: 1->21
pBR322 tet promoter and message start: 22->56
aphA-2: 90->884
UR1: 885->905.
1 CACCAAACAC CCCCCAAAAC CTTGACAGCT TATCATCGAT AAGCTTTAAT
51 GCGGTAGTTT AGGTGAGGAC AGGATGAGGA TCGTTTCGCA TGATTGAACA
101 AGATGGATTG CACGCAGGTT CTCCGGCCGC TTGGGTGGAG AGGCTATTCG
151 GCTATGACTG GGCACAACAG ACAATCGGCT GCTCTGATGC CGCCGTGTTC
201 CGGCTGTCAG CGCAGGGGCG CCCGGTTCTT TTTGTCAAGA CCGACCTGTC
251 CGGTGCCCTG AATGAACTGC AGGACGAGGC AGCGCGGCTA TCGTGGCTGG
301 CCACGACGGG CGTTCCTTGC GCAGCTGTGC TCGACGTTGT CACTGAAGCG
351 GGAAGGGACT GGCTGCTATT GGGCGAAGTG CCGGGGCAGG ATCTCCTGTC
401 ATCTCACCTT GCTCCTGCCG AGAAAGTATC CATCATGGCT GATGCAATGC
451 GGCGGCTGCA TACGCTTGAT CCGGCTACCT GCCCATTCGA CCACCAAGCG
501 AAACATCGCA TCGAGCGAGC ACGTACTCGG ATGGAAGCCG GTCTTGTCGA
551 TCAGGATGAT CTGGACGAAG AGCATCAGGG GCTCGCGCCA GCCGAACTGT
601 TCGCCAGGCT CAAGGCGCGC ATGCCCGACG GCGAGGATCT CGTCGTGACC
651 CATGGCGATG CCTGCTTGCC GAATATCATG GTGGAAAATG GCCGCTTTTC
701 TGGATTCATC GACTGTGGCC GGCTGGGTGT GGCGGACCGC TATCAGGACA
751 TAGCGTTGGC TACCCGTGAT ATTGCTGAAG AGCTTGGCGG CGAATGGGCT
801 GACCGCTTCC TCGTGCTTTA CGGTATCGCC GCTCCCGATT CGCAGCGCAT
851 CGCCTTCTAT CGCCTTCTTG ACGAGTTCTT CTGAGTGGTG TGGTGTGGTT
901 GTGTG
Last Update: Wednesday October 04 2017
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Eric Kofoid
eckofoid at ucdavis.edu