Universal Spectinomycin Resistance Cassette
(Standard template in strain TT24693)
Sequence of universal spec-cassette template.
Made by precise replacement of cat gene of uni-cmr-cassette with
spectinomycin resistance gene, aadA. Use strain TT24693 as
template for this cassette.
UL1 1 21
promoter 214 240
potential msg start 247
aadA (specR) 307 1317
UR1 1555 1575
1 CACCAAACAC CCCCCAAAAC CAAGCCACTG GAGCACCTCA AAAACACCAT
51 CATACACTAA ATCAGTAAGT TGGCAGCATC ACCCGACGCA CTTTGCGCCG
101 AATAAATACC TGTGACGGAA GATCACTTCG CAGAATAAAT AAATCCTGGT
151 GTCCCTGTTG ATACCGGGAA GCCCTGGGCC AACTTTTGGC GAAAATGAGA
201 CGTTGATCGG CACGTAAGAG GTTCCAACTT TCACCATAAT GAAATAAGAT
251 CACTACCGGG CGTATTTTTT GAGTTATCGA GATTTTCAGG AGCTAAGGAA
301 GCTAAAATGC GCTCACGCAA CTGGTCCAGA ACCTTGACCG AACGCAGCGG
351 TGGTAACGGC GCAGTGGCGG TTTTCATGGC TTGTTATGAC TGTTTTTTTG
401 GGGTACAGTC TATGCCTCGG GCATCCAAGC AGCAAGCGCG TTACGCCGTG
451 GGTCGATGTT TGATGTTATG GAGCAGCAAC GATGTTACGC AGCAGGGCAG
501 TCGCCCTAAA ACAAAGTTAA ACATCATGAG GGAAGCGGTG ATCGCCGAAG
551 TATCGACTCA ACTATCAGAG GTAGTTGGCG TCATCGAGCG CCATCTCGAA
601 CCGACGTTGC TGGCCGTACA TTTGTACGGC TCCGCAGTGG ATGGCGGCCT
651 GAAGCCACAC AGTGATATTG ATTTGCTGGT TACGGTGACC GTAAGGCTTG
701 ATGAAACAAC GCGGCGAGCT TTGATCAACG ACCTTTTGGA AACTTCGGCT
751 TCCCCTGGAG AGAGCGAGAT TCTCCGCGCT GTAGAAGTCA CCATTGTTGT
801 GCACGACGAC ATCATTCCGT GGCGTTATCC AGCTAAGCGC GAACTGCAAT
851 TTGGAGAATG GCAGCGCAAT GACATTCTTG CAGGTATCTT CGAGCCAGCC
901 ACGATCGACA TTGATCTGGC TATCTTGCTG ACAAAAGCAA GAGAACATAG
951 CGTTGCCTTG GTAGGTCCAG CGGCGGAGGA ACTCTTTGAT CCGGTTCCTG
1001 AACAGGATCT ATTTGAGGCG CTAAATGAAA CCTTAACGCT ATGGAACTCG
1051 CCGCCCGACT GGGCTGGCGA TGAGCGAAAT GTAGTGCTTA CGTTGTCCCG
1101 CATTTGGTAC AGCGCAGTAA CCGGCAAAAT CGCGCCGAAG GAGGTCGCTG
1151 CCGACTGGGC AATGGAGCGC CTGCCGGCCC AGTATCAGCC CGTCATACGT
1201 GAAGCTAGAC AGGCTTATCT TGGACAAGAA GAAGATCGCT TGGCCTCGCG
1251 CGCAGATCAG TTGGAAGAAT TTGTCCACTA CGTGAAAGGC GAGATCACCA
1301 AGGTAGTCGG CAAATAATTT TTTTAAGGCA GTTATTGGTG CCCTTAAACG
1351 CCTGGTGCTA CGCCTGAATA AGTGATAATA AGCGGATGAA TGGCAGAAAT
1401 TCGAAAGCAA ATTCGACCCG GTCGTCGGTT CAGGGCAGGG TCGTTAAATA
1451 GCCGCTTATG TCTATTGCTG GTTTACCGGT TTATTGACTA CCGGAAGCAG
1501 TGTGACCGTG TGCTTCTCAA ATGCCTGAGG CCAGTTTGCT CAGGCTCTCC
1551 CCGTGTGGTG TGGTGTGGTT GTGTG
Last Update: Wednesday October 04 2017
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Eric Kofoid
eckofoid at ucdavis.edu