Peptide cleaving enzymes and reagents used by PEPTIDESORT. Name Offset Specificity Overhang Documentation .. !Armillaria 0 '(C,K) . ! ArmillariaMellea 0 'K . ! AspN 0 'D . ! Endoproteinase Asp-N Chymo 1 (F,W,Y,M,L)'~P . ! Chymotrypsin Clos 1 R' . ! Clostripain CnBr 1 M' . ! Cyanogen Bromide IBzO 1 W' . ! IodosoBenzoate LysC 1 K' . ! Endoproteinase Lys-C Myxo 1 K' . ! Myxobacter Protease,Armillaria Protease NH2OH 1 N'G . ! Hydroxylamine NTCB 0 'C . ! NTCB + Ni pH2.5 1 D'P . ! pH 2.5 PancreaticElastase 1 (A,G,S,V)' . ! ProEn 1 P' . ! Proline Endopeptidase Staph 1 E' . ! Staphylococcal Protease Thermolysin 0 (L,I,M,F,W,V) . ! Trypsin 1 (K,R)'~P . ! Trypsin TrypK 1 K'~P . ! Trypsin(Arg blocked) TrypR 1 R'~P . ! Trypsin(Lys blocked) TrypsinCys 1 (K,R,C)~P . ! Trypsin (cys modified) V8AmoniumAcetate 1 E'~P . ! Staphylococcus Aureus V8 NoCut 1 @' . ! for calculations on whole peptide