1.

Pellet 1 mL fresh o/n

Resuspend in 0.5 mL "QDNA" buffer

(Vortexing at this point will cause shearing,

which may or may not be bad, depending on your needs).

5' x 100C

2.

Spin down debris, save the supernatant as a

frozen stock.

3.

Use only after a dilution of at least 1:100, or the

SDS in the buffer will inhibit subsequent reactions; do

not let the DNA sit for long periods of time after dilution,

as it is heavily contaminated with nucleases.

	10	mM	Tris [pH 7.4]
	1	mM	EDTA
	0.2	%	SDS