Common Prehybridization Solutions

Jeff Lawrence (Z10)

SDS Prewash:

	50 mM	Tris, pH 8.0	5 mL	1 M	Tris, pH 8.0
	1 M	NaCl		20 mL	5 M 	NaCl
	1 mM	EDTA		200 mL	500 mM 	EDTA, pH 8.0
	0.1%	SDS		1 mL	10%	SDS
				74 mL		ddH₂O

Poly-T Prewash:

	50%		Formamide	
	5 X		Denhardts	
	5 X		SSPE	
	0.1%		SDS
	100 µg/mL	Denatured Salmon Sperm DNA

Formamide Prewash:

	50%	Formamide	
	0.1%	SDS
	600 mM	NaCl
	80 mM	Tris, pH 8.0
	4 mM	EDTA

Sarkosyl Prewash:

	1 X		HPB
	1%		Sarkosyl
	100 µg/mL	Denatured Salmon Sperm DNA

SSC Prewash:

	6.0 X		SSC
	0.5%		SDS
	5.0 X		Denhardts
	100 µg/mL	Denatured Salmon Sperm DNA

HPB Hybridization:

	1 X	HPB		20 mL	5X 	HPB
	1%	Sarkosyl	10 mL	10%	Sarkosyl
				70 mL		ddH₂O

Last Update: Thursday June 19 2014

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